The affinity constant of soluble antibody was measured by non-competitive ELISA. 免疫细胞化学检测其抗原结合活性,非竞争ELISA法检测其亲和常数。
Determination of Affinity Constant of Anti-rHu-IFN-α McAb 抗rHu-IFN-αMcAb亲和常数的测定
AIM: To purify and refold the inclusion body of a human anti-HBsAg scFv with a 6 × His tag, and to determine the affinity constant of the purified recombinant product. 目的:对融合6×His标签的人源抗乙型肝炎表面抗原单链抗体的包涵体进行纯化和复性,并对复性产物的抗原结合性质进行鉴定。
There was insignificant difference upon the affinity constant of high and low affinity EIR on erythrocytes membrane in rats between mild injury group and severe injury group. 轻度损伤组和重度损伤组大鼠红细胞膜高、低胰岛素受体亲和常数比较,差异均无统计学意义。
Measurement of affinity constant of monoclonal antibody by competitive antibody binding to antigen 以抗体竞争结合抗原测定单抗亲和力常数的研究
Measurement of Affinity Constant of Humanized Monoclonal Antibody 813-F ( ab)_2 Binding to Platelets of Human, Macaque and Dog 人源化单抗813-F(ab)2与人、猕猴、毕格犬血小板反应亲和常数的测定
Expression of Calmodulin binding Domain of Neuronal Nitric Synthase and Its Binding Activity to Calmodulin The interaction between calmodulin and calmodulin binding domain of cell membrane can be characterized by affinity constant. 脑型一氧化氮合成酶的钙调蛋白结合区的表达及活性鉴定钙调素(Calmodulin,CaM)是一种多功能的酶调节物。
Measurement of Affinity Constant in Wastewater Bio-treatment 废水生物处理中基质亲和性常数的测定方法探讨
The labelling efficiency, IRF, radiochemical purity, affinity constant and stability in vitro were determined. 方法:采用NBS法进行碘标,并测定标记物的标记率、免疫活性分数、放化纯度、亲和常数和体外稳定性。
Objective To develop a more reliable and stable method for determining monoclonal antibody ( mAb) affinity constant based on competitive antibody/ antigen binding. 目的在Friguent方法基础上建立更加稳定可靠的基于抗原/抗体竞争结合原理的单抗亲和力常数(Kd)检测方法。
Optimized expression of a single-chain Fv antibody against human asialoglycoprotein receptor and determination of its affinity constant 去唾液酸糖蛋白受体特异性单链抗体的优化表达及亲和常数的测定
Methods Affinity constant of the specific antibodies was determined with ELISA and capillary electrophoresis. 方法采用ELISA和毛细管电泳测定抗体抗原的亲和常数。